FastQCFastQC Report
Thu 26 May 2016
SRR1781484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179184
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT11930366.58127957853381No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG105525.888918653451201No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC47222.6352799357085455No Hit
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT25611.4292570765246897No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT18981.0592463612822574Illumina PCR Primer Index 6 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC16300.9096794356638985No Hit
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT15620.8717296187159569No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC14160.7902491293865523No Hit
CATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT9360.5223680685775516No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG8840.4933476203232432No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC7860.4386552370747388No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC7040.3928922225198679No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC5910.32982855612108225No Hit
CTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG5100.28462362710956335No Hit
TATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCT4740.26453254754888833Illumina PCR Primer Index 6 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG4590.25616126439860704No Hit
CTAAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3680.2053754799535673No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAAAATCTCGTATGCCGT3660.2042593088668631No Hit
TCTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG3400.18974908473970892No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT3350.18695865702294848No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC2940.164077149745513No Hit
CAAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT2820.157380123225288No Hit
CTTATACACATCACCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT2270.12668541834092328No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2100.11719796410393786No Hit
CTTATACACATCTCCGAGCCAACGAGACGATCCAAAATCTCGTATGCCGT2090.11663987856058576No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGACGT2070.1155237074738816No Hit
TCATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG2030.11329136530047325No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCAGT1990.11105902312706492No Hit
CATAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT1920.10715242432360034No Hit
CTTATACACATCTCCGAGCCCACGAGACGCTCCAAAATCTCGTATGCCGT1890.10547816769354407No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA252.8237027E-670.0312739
ACACGTA252.8237027E-670.0312738
ACTCGAA401.3278623E-1070.0312738
AATCTCT150.00221789770.0312736
AATCTAT150.00221789770.0312736
CACGTAA150.00221789770.0312739
TCGAAAG252.8237027E-670.0312740
ACGTAAG207.8929E-570.0312740
AACTCGA401.3278623E-1070.0312737
AACACGT252.8237027E-670.0312737
CAACATC252.828392E-670.0117333
AGTATGA301.0181793E-770.0117341
CGAAAGC301.0181793E-770.0117341
TCCAATA150.002220355470.0117331
CCAACAT207.903828E-570.0117232
ATGCCCT207.903828E-570.0117244
ATGCAGA207.903828E-570.0117244
AAAGCCG207.903828E-570.0117243
GAAAGCC207.903828E-570.0117242
TTAGACA150.002222815869.992192
Produced by FastQC (version 0.11.5)