FastQCFastQC Report
Thu 26 May 2016
SRR1781483_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781483_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195399
Sequences flagged as poor quality0
Sequence length76
%GC97

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG17618390.16576338671129No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2300.11770786953873869No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2080.10644885593068543No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGGG150.002218578270.0282111
TGTGGCG150.002218578270.0282111
TGCCGGC353.6870915E-970.01025419
CTGCCGG353.6870915E-970.01025418
GCCGGCG455.456968E-1269.9923220
CCGGCGG252.8340019E-669.9923121
CCGGCGA207.916455E-569.9923121
CGTCTGG207.9265155E-569.974379
CGGGGCT207.946667E-569.938527
GTGGGTC150.002229886869.9385238
GCGTCTG207.946667E-569.938528
CTTGTGC207.946667E-569.938521
TTGTGCG207.946667E-569.938522
CGCGTCT207.946667E-569.938527
TGCGCGT207.956758E-569.9206165
CATCCAT150.002232153769.9206165
GCGCGTC150.002232153769.9206166
GGGTGTA150.002232153769.92061634
GTGCGCG252.851315E-669.920614
TGTGCGC150.002234422469.902723
Produced by FastQC (version 0.11.5)