FastQCFastQC Report
Thu 26 May 2016
SRR1781483_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781483_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195399
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT12834165.681502975962No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG114655.867481409833213No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC53342.729799026607096No Hit
CTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT20231.0353174785950798No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT19821.0143347714164352Illumina PCR Primer Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC19450.9953991576210728No Hit
CTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC15590.7978546461343201No Hit
CTTAAACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT13070.6688877629875281No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG9280.47492566492151955No Hit
CATATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT8090.4140246367688678No Hit
TTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC7460.3817829159821698No Hit
CTTTACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC6450.3300938080542889No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG5520.28249888689297287No Hit
TATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCT4950.2533278061811985No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGACATCTCGTATGCCGT4750.2430923392647864No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG4310.2205743120486799No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC4010.2052211116740618No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCACGTATGCCGT3810.19498564475764973No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT3500.17912067103721105No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT3430.17553825761646683No Hit
TCTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG3050.15609087047528392No Hit
CTAAAACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT2820.14432008352141004No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTAAGCCGT2770.14176121679230702No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCGGTCATCTCGTATGCCGT2740.1402258967548452No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTAGTATGCCGT2580.13203752322171555No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2180.11156658938889145No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCAGT2090.10696062927650603No Hit
CTTATACACATCTCCGAGCCAACGAGACAAACGGTCATCTCGTATGCCGT2030.1038899892015824No Hit
TCATATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG1960.10030757578083818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTAA455.456968E-1270.0404839
ACACGTA301.01576006E-770.04047438
CTCCAAA150.00221704370.04047439
TCTACTA150.00221704370.04047438
GGTCAAA301.01576006E-770.04047433
CGGAAAT150.00221704370.04047432
TCACGAA353.6761776E-970.04047438
ATAGTAT353.6761776E-970.04047439
GACAACT150.00221704370.04047434
ATCACGA353.6761776E-970.04047437
TCCAAAG150.00221704370.04047440
ATCGTAA252.822455E-670.04047439
TATAGTA301.01576006E-770.04047438
CATATAG353.6761776E-970.04047436
CTACTAT150.00221704370.04047439
CGGTAAA150.00221704370.04047432
ATATAGT301.01576006E-770.04047437
CGTTCAT207.8895486E-570.0404732
AAATCGT207.8895486E-570.0404737
GGACAAC207.8895486E-570.0404733
Produced by FastQC (version 0.11.5)