FastQCFastQC Report
Thu 26 May 2016
SRR1781482_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781482_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136562
Sequences flagged as poor quality0
Sequence length76
%GC92

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11182381.88441879878737No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG43573.1904922306351695No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT3290.2409162138808746No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGG1500.1098402190946237No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCTGT150.002213181270.0597963
GCCGGCG150.002219631370.0084220
TTTTATC150.002222861669.9827826
CTTTTAT150.002229332769.931521
GTAGGAC150.002229332769.931523
AAAAAGG3800.064.52875570
TTTATAC352.9765215E-759.941313
TGGTCGC5450.057.16284649
TCGCCGT5400.057.06482752
GCCGTAT5400.057.06482754
CGCCGTA5400.057.06482753
CCGTATC5350.056.9436255
TCGGTGG5550.056.7220145
ATCTCGG5500.056.6016942
TCTCGGT5500.056.6016943
GATCTCG5500.056.6016941
CTCGGTG5500.056.6016944
GTCGCCG5450.056.54129451
GGTCGCC5450.056.5205750
GTATCAT5400.056.41636357
Produced by FastQC (version 0.11.5)