FastQCFastQC Report
Thu 26 May 2016
SRR1781482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136562
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT7768456.885517200978306No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG77865.701439639138267No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55544.067017179010266No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC21531.5765732780714987No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC20011.46526852272228No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT15881.162841786148416No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT14431.05666290769028TruSeq Adapter, Index 11 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC12610.9233901085221364No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG8100.5931371831109679No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT7250.5308943922906811No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT5430.3976215931225377No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC5410.3961570568679428No Hit
CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT5230.3829762305765879No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATCTCGTATGCCGTCT4990.36540179552144814RNA PCR Primer, Index 38 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC4870.35661457799387825No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG4080.29876539593737644No Hit
TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT3760.2753328158638567RNA PCR Primer, Index 11 (95% over 21bp)
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT3460.25336477204493196No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC3260.23871940949898215No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCAGT3090.2262708513349248No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2820.2064996118978925No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAG2720.19917693062491762No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT2460.18013795931518284No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAATCTCGTATGCCGTC2050.1501149660959857RNA PCR Primer, Index 29 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG2030.14865042984139074No Hit
TCTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT1990.14572135733220076No Hit
GTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT1940.1420600166957133No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTAAGCAGT1860.13620187167733339No Hit
CTTATAAACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT1590.11643063224030112No Hit
CTTATACACATCACCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT1480.10837568284002871No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT1460.10691114658543373No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATAC353.6652636E-970.039951
CTTTACA455.456968E-1270.039941
CTTAAAC455.456968E-1270.039941
AATATAC301.0135227E-770.039941
TCTTAAT301.0135227E-770.039941
AAACTCG207.8956684E-570.0142936
ACTCGAA207.8956684E-570.0142938
GAATGCA207.8956684E-570.0142942
AACTCGA207.8956684E-570.0142937
ACACAAA207.8956684E-570.014296
AGATATA353.6743586E-970.0142931
TATACAA301.0161057E-770.014283
TCCTAGA150.00221889970.0142855
TATCGAA150.00221889970.0142838
AACTAGT150.00221889970.0142837
ACGGGGG150.00221889970.0142870
ATGCATG150.00221889970.0142854
CAGATAG150.00221889970.0142854
ACTAGTA150.00221889970.0142838
ACGAATG150.00221889970.0142840
Produced by FastQC (version 0.11.5)