FastQCFastQC Report
Thu 26 May 2016
SRR1781481_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781481_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437579
Sequences flagged as poor quality0
Sequence length76
%GC74

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21447949.014920734313115No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG46801.0695211607504018No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGTA150.00222046270.0279353
TCGACGT150.002221470770.0199252
CTGCCGT150.002229551669.9558540
GCCGTAT6750.056.02876754
TATCGGT252.3968518E-455.96468743
CGCCGTA6800.055.6104253
TCGCCGT6800.055.60405352
GTGGTCG7050.055.593248
CGTATCA6900.055.33092556
TGGTCGC7050.054.61296549
TCGGTGG7250.054.52355645
CCGTATC6900.054.3032455
GTCGCCG7050.054.12267751
GTATCAT7200.053.51194457
ATCTCGG7000.053.4662642
GATCTCG7000.053.4662641
GGTCGCC7200.052.98905650
TCTCGGT7100.052.7132143
GCGTCAA200.006960228652.478897
TCATTAA7450.052.19236460