FastQCFastQC Report
Thu 26 May 2016
SRR1781481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences437579
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT17062438.99273045552917No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG128172.9290710934482687No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC77241.7651669755632697No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG60391.3800936516606144No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC25140.5745248286595106No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT20140.4602597473827583TruSeq Adapter, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC14460.33045461505236773No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT12620.28840506514252284No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG9950.2273875117407371No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT8340.19059415556962286No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC6390.14603077387168947No Hit
CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT6130.1400889896452983No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC5840.1334616149312467No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG5060.11563626225207335No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG5010.11449361143930582No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAACAA150.00222349170.0038865
TACTACG150.00222349170.0038842
TGTCCGA207.929931E-569.9878911
AGGGGGG198950.067.2417370
TCGGAAC700.065.0110433
AAGGGGG212650.062.87675569
GAATTCG750.060.67003629
AAAGGGG222500.060.1561568
AAAAGGG223250.060.03244867
AAAAAGG223800.059.94747566
AAAAAAG224600.059.87420365
CGTAATC1350.059.63294228
GACGAAT1000.059.49650226
TATGACG1300.059.2340643
CCGTCTT218450.059.1884547
CGTCTTC219550.059.17887548
GCCGTCT218800.059.1737646
ATGCCGT219150.059.15911544
TGCCGTC219200.059.1296545
TATGCCG219450.059.12608743