FastQCFastQC Report
Thu 26 May 2016
SRR1781480_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781480_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352281
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19025354.00603495505008No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG46301.3142917159880891No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT3630.10304274144787827No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATAG207.8928904E-570.0502255
AACGATA150.002217300270.0502254
CGTATCA6150.057.52091256
TCGCCGT6150.057.49637252
CGCCGTA6150.057.49637253
CCGTATC6200.057.05703755
GCCGTAT6200.057.05703754
GTCGCCG6200.057.02458651
GTGGTCG6250.055.99238248
TCTCGGT6200.055.86361743
TGGTCGC6300.055.55589349
GTATCAT6400.055.27457
CGGTGGT6400.055.2190146
ATCTCGG6400.055.179842
GATCTCG6450.054.7520541
TCATTAA6500.054.43137760
CTCGGTG6450.054.24076544
GTAGATC6600.054.0374638
GGTCGCC6550.053.96979550
TCGGTGG6650.053.6616545
Produced by FastQC (version 0.11.5)