FastQCFastQC Report
Thu 26 May 2016
SRR1781480_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781480_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences352281
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT15106942.88309616470942No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG111853.175022212381593No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC67071.9038778702229187No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59831.6983601159301809No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC21270.6037793693102949No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT16640.472350197711486TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC11180.31736028908740466No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT10580.30032843099684625No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC8940.25377468554932003No Hit
CTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT8430.23929760617234538No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG8390.23816214896630813No Hit
CATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT5380.1527189942120069No Hit
CTTTACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC5200.14760943678483937No Hit
TTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC4830.13710645762899504No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG4650.13199690020182753No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT3960.11241026339768537No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAG150.002225122669.98807520
CACGATA150.002225122669.98807521
GCCGACG150.002225122669.98807518
GTCGTGT150.002225122669.98807521
CGATACG150.002225122669.98807523
AGTCGAG150.002225122669.98807526
AGGGGGG174550.067.50210670
AGCCGTC550.063.62552645
TGCCGAC501.4551915E-1162.9892745
AAGGGGG189400.062.26569
AATCACG453.8198777E-1062.24696436
ACTCCGA800.061.2482611
CACACGA800.061.23956319
TCTACGA750.060.66494411
AAAGGGG198750.059.33580468
AAAAGGG199350.059.28009467
AAAAAGG200000.059.1574266
AAAAAAG200950.058.98223565
GCCGTCT195400.058.90245446
CCGTCTT194450.058.90228347
Produced by FastQC (version 0.11.5)