FastQCFastQC Report
Thu 26 May 2016
SRR1781479_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781479_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292032
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21660474.17132369055446No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG45461.5566787201402585No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT3540.12121959237343853No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGGC207.912282E-570.0114919
TGAGCGA150.002221789470.0114919
GCTCACC150.002221789470.0114921
CCGGCGG453.8380676E-1062.23243321
AAAAAGG5950.060.0510270
CTTGGTG252.3962602E-455.961223
CGTATCA7350.055.7425456
CCGTATC7350.055.7425455
TCGCCGT7600.055.2722252
GCCGTAT7450.054.9943254
TGGTCGC7650.054.89214749
GATCTCG7650.054.8639441
CGCCGTA7600.054.83042553
CGGTGGT7850.054.82156846
TGTAGAT7850.054.80278437
GTGGTCG7800.054.73380748
GTCGCCG7750.054.64475651
ATCTCGG7700.054.50768342
GGTGGTC7850.054.37586247
GGTCGCC7800.054.28516850
Produced by FastQC (version 0.11.5)