FastQCFastQC Report
Thu 26 May 2016
SRR1781479_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781479_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292032
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT16807757.55430911680912Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG128224.390614727153189No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC66032.2610535831689678No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59032.0213538242384397No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC27240.9327744904667982No Hit
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT26300.9005862371246987Illumina PCR Primer Index 6 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC17840.6108919570458032Illumina PCR Primer Index 6 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG12510.4283777120315582No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT12100.41433815472277014Illumina PCR Primer Index 6 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT8620.29517313171159326Illumina PCR Primer Index 6 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC7570.259218167872014Illumina PCR Primer Index 6 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC7470.2557938856015779Illumina PCR Primer Index 6 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATCTCGTATGCCGTCT7380.2527120315581854TruSeq Adapter, Index 4 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG6060.20751150558842865No Hit
TATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCT5790.19826594345825113Illumina PCR Primer Index 6 (95% over 23bp)
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT5340.18285667324128863Illumina PCR Primer Index 6 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG5330.182514245014245No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC5030.17224139820293666No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT4740.1623109796186719TruSeq Adapter, Index 6 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT4290.1469017094017094Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAATCTCGTATGCCGTC3430.11745288187595879No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCAGT3310.11334374315143546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTCG550.070.00822436
ACCGGCA500.070.0082227
CACGAAA150.002222202370.0082239
ACGGCCA207.914124E-570.0082228
ACTCGTA500.070.0082238
TCGTATC150.002222202370.0082240
ACTCGAA353.6980055E-970.0082238
AACTCGA353.6980055E-970.0082237
AAATCCG207.9208425E-569.9962310
AAGACGT252.836592E-669.9962344
ACCGAGA150.002223712469.9962313
AGACGTC150.002223712469.9962345
GCCGACA207.9208425E-569.9962346
GAAGTCT207.9208425E-569.9962346
GTCCGAG150.002223712469.9962312
CGAGCAA207.9208425E-569.9962315
ACCACGA750.069.9962319
CCGACAT150.002223712469.9962347
TATAGAC252.836592E-669.996233
ACGTATT150.002223712469.9962347
Produced by FastQC (version 0.11.5)