FastQCFastQC Report
Thu 26 May 2016
SRR1781478_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781478_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331554
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20986463.29707981203665No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG50081.5104628506970208No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT3940.11883433769461385No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGGC150.002220587870.0231311
ACTCGAG150.002220587870.0231310
GGAAACG150.002221919870.012559
TACTCGA150.002221919870.012559
CGAGCAC150.002223252670.0019625
TTGTGCG150.002237951469.885772
GTCGGTG308.3956E-658.29972544
TGTCGGT252.3953534E-455.9677343
GCGGTGA200.006922027652.5491359
AGCCGTG200.0069261752.5411862
TAATGCG200.0069261752.5411855
CTCGAGA200.0069386152.5173511
AATTGAG200.006942760752.5094120
CCCGTGA200.00694691352.50147224
TCGAGGA200.006951066752.49353846
CGATACT200.00698019752.4380654
TGTGCGG200.006984365652.430153
TACGTAC200.00699270952.4143261
CCGTATC6950.051.91119455
CGTATCA7050.051.6795256
Produced by FastQC (version 0.11.5)