FastQCFastQC Report
Thu 26 May 2016
SRR1781478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331554
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT16562649.954456890883534No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG126133.8042068561983866No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC71602.1595275581051654No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64501.9453844622595413No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC25210.7603587952490394No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT24230.7308010158224603RNA PCR Primer, Index 37 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC16160.48740175054440604No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT11710.3531853031482051No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG10590.3194049838035433No Hit
CTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT9910.2988955042014272No Hit
CTTTACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC7420.22379461565838446No Hit
TTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC6680.20147547609137575No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT6570.19815776615573935No Hit
CTTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG5300.15985329689884603No Hit
TATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCT5120.15442431700416825RNA PCR Primer, Index 37 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG5100.1538210970158707No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT4440.13391483740205215No Hit
TCTTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC4130.12456492758344041No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTAAG252.8310187E-670.0229440
AACTCGA150.002220617170.02293437
AATGACG207.9133795E-570.0123743
AAGCCGA207.919295E-570.0018144
AAATCGG150.00222460769.9912568
GTCCGAG150.00222460769.9912512
ATACGAG207.9252146E-569.9912512
AGGGGGG188200.067.16482570
CCGACTT453.8380676E-1062.22382747
ATCTACG453.8380676E-1062.21444710
TCTACGA453.8380676E-1062.21444711
CTACGAG453.8380676E-1062.21444712
ATGACGT1300.061.9246844
AAGGGGG204500.061.7942369
TATGACG1200.061.26082243
CGAGCAC1100.060.44699515
TCACGTA700.060.01965738
TCGTAAG650.059.25017540
AAAGGGG215550.058.6913468
AAAAGGG215950.058.5988367
Produced by FastQC (version 0.11.5)