FastQCFastQC Report
Thu 26 May 2016
SRR1781477_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781477_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264536
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23244987.87045997520187No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG56062.1191822663077993No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT4630.17502343726373726No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTGCC207.89911E-570.0325716
CGGACGC150.00221890570.0325612
AACCCCA150.002220572270.0193153
CGTCTGG301.02061676E-770.006059
CCGGCGA150.002222240270.0060521
CTTTTCC150.002223909469.99280525
GTGATGC150.002227250569.9663241
GGGATGG150.002227250569.9663241
GTAGATG150.00223226969.92663638
TTACACA150.00223226969.9266363
TTTACAC207.96603E-569.913422
TTGTGCG150.002233943769.9134142
AAAAAGG5600.063.178370
GCGTCTG401.0622898E-861.2553028
GCCGGCG601.8189894E-1258.3383820
TGCGCGT308.395618E-658.2942435
CATTAAA7250.057.0136161
CGTATCA7300.056.6123956
TATCATT7250.056.53044558
GTATCAT7250.056.5197457
Produced by FastQC (version 0.11.5)