FastQCFastQC Report
Thu 26 May 2016
SRR1781477_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781477_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264536
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT17852567.48608884991079No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG137785.2083648350319045No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG71612.7070039616536126No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC68332.583013276075846No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC29671.121586475942783No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT25400.960171772461971TruSeq Adapter, Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC20960.7923307224725556No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT16790.6346962228203344No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT13340.5042791907339644No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG13110.4955847219282064No Hit
TTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC9960.3765083013276076No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC9510.35949738409895066No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT8750.3307678350016633No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT8610.32547554964163666No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT8580.32434148849305955No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG6260.23664075966976142No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT6190.2339946169897481No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC5460.20639912904103788No Hit
CATATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT5300.2003508029152932No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG5040.19052227296095806No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT4420.16708500922369734No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACATCTCGTATGCCGTC4330.16368282577796595No Hit
GTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT2810.1062237275833913No Hit
TCTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT2780.10508966643481416No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACGC455.456968E-1270.018163
TATACAA353.6907295E-970.018163
TTATACG455.456968E-1270.018162
GTTATAC353.6907295E-970.018161
ATCTTAT150.002220719870.018151
TAAAACA301.01939804E-770.018152
CTCTATA150.002220719870.018151
CTAAAAC301.01939804E-770.018151
TCTCATA150.002220719870.018151
CAAAAAC207.907189E-570.018151
AAGTATG252.8337636E-670.0049240
AGCCGTA252.8337636E-670.0049245
CCGACTT353.6961865E-970.0049247
GCCGACT353.6961865E-970.0049246
CATATAG252.8337636E-670.0049236
GCCCAAC150.002222386270.0049133
GCAAGAA207.9146E-570.0049156
TAAGCTT150.002222386270.0049153
CAACTCG600.070.0049136
ACCAACT455.456968E-1270.0049134
Produced by FastQC (version 0.11.5)