FastQCFastQC Report
Thu 26 May 2016
SRR1781476_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781476_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263838
Sequences flagged as poor quality0
Sequence length76
%GC95

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23249888.12149879850514No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG61492.330596805615567No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT5390.20429202768365434No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATAC207.876768E-570.07255662
ACAGGAC207.876768E-570.07255667
GAAAGGG150.002213880470.07255669
AGCTCAG207.913898E-570.0060722
TGCCGGC252.833458E-670.0060719
TGCCGAG150.002222232570.0060719
TCTGGCG252.833458E-670.0060711
GCTGGCG150.002222232570.0060711
CTGGCGC353.6961865E-970.0060712
GCGCTGC207.913898E-570.0060715
CGGTGCC150.002222232570.0060716
TGTGGCG207.913898E-570.0060711
AATACTT150.002222232570.0060721
CTGCCGG301.02061676E-770.0060718
TGGCGGT252.833458E-670.0060713
TGGCGCT207.913898E-570.0060713
GGTGCCG150.002222232570.0060717
GGTCTGG150.002223905669.992799
CGGGTGT150.002223905669.992797
CGGGTCT150.002223905669.992797
Produced by FastQC (version 0.11.5)