FastQCFastQC Report
Thu 26 May 2016
SRR1781476_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781476_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences263838
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT18660370.72635480863258No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG139475.286198348986878No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79603.017002857814265No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC73782.796412950371061No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC24730.9373175964038539No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT19570.7417430392892608RNA PCR Primer, Index 36 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC16630.6303110241890857No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT10840.4108581781244552No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG10210.3869798891744176No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT9790.37106102987439266No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT8210.31117579726953665No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC7640.2895716310766455No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT6660.2524276260432538No Hit
TTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC6610.2505325237456318No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG5810.22021088698367935No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG5530.20959831411699603No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACATCTCGTATGCCGTC4730.17927667735504363No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT4600.17434941138122637No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC4540.17207528862407995No Hit
TATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCT4510.17093822724550672No Hit
GTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT2660.10081944223349178No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGACGT2650.10044042177396735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTAT353.6925485E-970.015921
TATATAC252.8310951E-670.015923
TTATACG252.8310951E-670.015922
TGATACA207.908394E-570.015922
TACAAAT455.456968E-1270.015925
CTTACAC150.002220993670.015921
GCGTCAG150.002220993670.015921
AATACAA207.908394E-570.015923
GTTATAC252.8310951E-670.015921
ACTGCTT600.070.00265553
CTCGAAT750.070.00265539
TTGTTTA207.9158264E-570.00265534
TTATGAT150.002222664770.00265552
ACACGTA150.002222664770.00265538
ATGGGGG301.0209624E-770.00265570
GGGCAAA207.9158264E-570.00265544
TGACTTG207.9158264E-570.00265530
ACCACTC207.9158264E-570.00265560
AGGACAC207.9158264E-570.00265555
TCGGCTT207.9158264E-570.00265553
Produced by FastQC (version 0.11.5)