FastQCFastQC Report
Thu 26 May 2016
SRR1781475_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781475_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261286
Sequences flagged as poor quality0
Sequence length76
%GC97

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23658990.54790536040966No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG62552.393928492150364No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT5100.19518841422808722No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGG2620.10027326377991931No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATTAAA207.8614474E-570.0998261
ATAATTA150.0022104470.0998259
TAATTAA150.0022104470.0998260
GTATAAT150.00221212470.08636557
CCGTATA150.00221212470.08636555
CGTATAA150.00221212470.08636556
CGCTGGG150.002213808770.0729216
GCGCTGG150.002213808770.0729215
CTGGCGC150.002217181370.04603612
TCTGGCG207.898905E-570.032611
TCGGGCG150.00221886970.032611
GTCGGGC150.002223937869.99232510
CGAGCTA150.00222901569.952125
TGCGGGT207.9591315E-569.9253165
AGGTGGT150.00223240569.9253146
CTTAATA150.002234101369.911931
GTTATGC150.002234101369.911931
TTATGCG150.002235798669.8985442
AATACAC150.002235798669.8985444
TTAATAC150.002235798669.8985442
Produced by FastQC (version 0.11.5)