FastQCFastQC Report
Thu 26 May 2016
SRR1781475_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781475_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences261286
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT18600271.1871282808876No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG143055.474843657907427No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG80183.068668049570203No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC68162.60863574780126No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC30861.1810812672703475No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT25440.9736457368553999RNA PCR Primer, Index 36 (95% over 23bp)
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC20390.7803709345315095No Hit
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT14680.5618364550722197No Hit
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT11050.4229082308275223No Hit
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG10880.4164019503532528No Hit
TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC8860.33909202942369665No Hit
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC8640.33067213704523013RNA PCR Primer, Index 36 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG7480.28627634086786125No Hit
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT6770.2591030518282648No Hit
CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG6260.2395842104054561No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT5970.22848526136111388RNA PCR Primer, Index 36 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACTAACAACATCTCGTATGCCGTC5660.21662086755509288No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC5320.20360830660655374No Hit
CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT5140.19671930375144475No Hit
CTTATACACATCTCCGAGCCAACGAGACTAACAACAATCTCGTATGCCGT3320.1270638304386764No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCAGT2910.11137221282426155No Hit
TCTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT2750.10524865473083135No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAAGTA301.0171789E-770.0398438
ACTCGAA207.894884E-570.0398438
TAACGTA207.894884E-570.0398438
AATCAAG301.0171789E-770.0398436
ACACTCA150.002217962970.0398434
CAATCAA301.0171789E-770.0398435
ATAACGT207.894884E-570.0398437
AACTCGA150.002217962970.0398437
CAATAAC207.894884E-570.0398435
AATATAG207.894884E-570.0398436
AATAACG207.894884E-570.0398436
TATAGTA150.002217962970.0398438
ATATAGT150.002217962970.0398437
CGTATCC150.002219648670.0264341
ATCGTAA150.002219648670.0264339
CAAAAAT650.070.0264332
AACGTAT252.8284958E-670.0264239
TCGTATC207.902379E-570.0264240
CAAGTAT207.902379E-570.0264239
CTGATAC150.002221335270.013021
Produced by FastQC (version 0.11.5)