FastQCFastQC Report
Thu 26 May 2016
SRR1781474_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781474_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678504
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19467628.69194581019419No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG37620.5544550953273673No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACTACG200.00697239952.458854
GCCGTAT6000.049.01581654
CGTATCA6050.048.03911656
TCGCCGT5950.047.65891652
CGCCGTA6050.047.45332753
CCGTATC6250.047.0586555
GTGGTCG6450.045.5725648
TGGTCGC6700.043.87856349
GTCGCCG6600.042.9588951
TCTCGGT6900.042.0777643
ATCATTA6950.041.824459
GTATCAT6950.041.82131657
TCGGTGG7200.041.78819745
CGGTGGT7400.041.1376346
GATCTCG7100.040.8894541
CTCGGTG7200.040.8103644
ATCTCGG7150.040.60351642
TCATTAA7250.040.57978460
CGTACCT350.0012593439.9745540
GGTCGCC7600.037.76140650
Produced by FastQC (version 0.11.5)