FastQCFastQC Report
Thu 26 May 2016
SRR1781474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678504
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT15092822.244231426785987Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG112991.6652812658436797No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC58760.8660228974331765No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG48180.7100916133139966No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC24890.3668364519590157No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT20970.30906228997913054Illumina PCR Primer Index 1 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC16530.2436242085529341Illumina PCR Primer Index 1 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT11510.16963790928277506Illumina PCR Primer Index 1 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG9910.14605661867874029No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT8270.12188579580960465TruSeq Adapter, Index 1 (95% over 23bp)
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT7750.11422187636329335Illumina PCR Primer Index 1 (95% over 21bp)
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC7180.10582104158560598Illumina PCR Primer Index 1 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG175150.066.2645370
CGATCAC453.8380676E-1062.2372335
AAGGGGG189500.061.24209269
AAAGGGG198550.058.5211668
AAAAGGG199150.058.45028767
ATGCCGT195450.058.3391544
GCCGTCT194900.058.3242246
CCGTCTT194850.058.3032647
TATGCCG195300.058.29865643
CGTCTTC195600.058.2944248
GCTTGAA196100.058.22146256
TCGTATG197000.058.1637740
CTCGTAT195900.058.15082639
TCTCGTA195950.058.08238638
TGCCGTC196100.058.07439445
CGTATGC197050.057.98056841
GTATGCC196450.057.97519742
GATCTCG196500.057.97326336
ATCTCGT195850.057.96904837
TGCTTGA196100.057.95800855
Produced by FastQC (version 0.11.5)