FastQCFastQC Report
Thu 26 May 2016
SRR1781473_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781473_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688011
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23319933.89466156791098No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG57370.833852947118578No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGGT200.00692833552.54309563
ATGCGTA200.006940314552.52014513
TACACGG200.00697033252.4628645
CGCCGTA8550.051.18922853
CGTATCA8700.050.31031456
GCCGTAT8750.050.01918454
CCGTATC8850.049.45455
GTATCAT9150.048.21872757
TGGTCGC9250.048.05150649
TCGCCGT9050.047.9672252
GTGGTCG9450.047.40144748
TCGGTGG9500.047.1348245
TCTCGGT9550.046.51495743
CGGTGGT9650.046.41227746
GGTGGTC9700.046.1730447
ATCTCGG9600.045.8983342
GATCTCG9700.045.4251541
GTCGCCG9650.044.9815351
CTCGGTG10000.044.4282544
ATCATTA10200.043.60467559
Produced by FastQC (version 0.11.5)