FastQCFastQC Report
Thu 26 May 2016
SRR1781473_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781473_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences688011
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT18163326.399723260238574No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG136601.9854333724315454No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC79261.1520164648530329No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74911.0887907315435363No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC28430.4132201374687323No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT24350.35391876001982525TruSeq Adapter, Index 25 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC18580.2700538218138954No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG12930.18793304176822756No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATCTCGTATGCCGTCT11600.1686019554919907No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT11320.1645322531180461No Hit
CTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT9730.14142215749457496No Hit
CTTTACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC7860.1142423594971592No Hit
TTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC7230.10508552915578384No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAACG150.002225082469.9955724
AGGGGGG213450.066.3228170
CGTATGA1600.061.25502441
AAGGGGG231050.061.22529669
AAAGGGG243650.058.05911668
AAAAGGG244600.057.8765567
AAAAAGG245250.057.72315266
AAAAAAG247100.057.5317765
TATCTCG239450.057.2817736
CCGTCTT236650.057.099747
ATATCTC240250.057.09102235
TATGCCG238900.057.03485543
CTCGTAT240850.057.02147739
CGTCTTC237100.057.0208548
ATGCCGT238950.056.98948744
GCCGTCT237550.056.98649246
TGCCGTC238400.056.9594845
TCGTATG241500.056.9508440
GCTTGAA240500.056.9423456
TGCTTGA238750.056.93461655
Produced by FastQC (version 0.11.5)