FastQCFastQC Report
Thu 26 May 2016
SRR1781472_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781472_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369810
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22132259.84748925123712No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG50851.375030421027014No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT4260.11519428895919526No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGGCG150.002220720570.02370511
CTGCGTC150.002220720570.02370513
CTACGTA150.002226692969.976327
CGCGTCT207.934798E-569.97637
ACGCATC150.00222788969.966846
GCGCGTC252.8451705E-669.966846
TGTGCGG150.002233875469.919523
TGCGCGT308.405899E-658.289925
AAAAAGG6450.054.31137570
CGTATCA7550.053.35099456
CCGTATC7450.053.1268255
GCCGTAT7500.052.76548454
GTCGCCG7700.052.73084351
GCGAAGC200.00692416152.5462570
CCCATTA200.00692416152.5462558
GTATCAT7600.052.5391357
TACTGCG200.00693902152.51777611
GCGTCTC200.00693902152.51777615
TGCGTCT200.00693902152.51777614
CGGAATT200.0069464652.5035518
Produced by FastQC (version 0.11.5)