FastQCFastQC Report
Thu 26 May 2016
SRR1781472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences369810
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT17336446.879208242070256No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG132603.5856250507017116No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC70031.8936751304724047No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66441.7965982531570266No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT25240.6825126416267814Illumina PCR Primer Index 6 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC25230.6822422324977692No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC19530.5281090289608177No Hit
ATTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT13730.37127173413374437No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC12420.33584813823314674No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG10240.27689894810848814No Hit
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT9690.2620264460128174No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC8330.22525080446715878No Hit
TTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC7820.21145993888753686No Hit
CATATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT6750.1825261620832319No Hit
CCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG5970.1614342500202807No Hit
CTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG5810.15710770395608556No Hit
TATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCT5620.15196993050485386Illumina PCR Primer Index 6 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC5010.1354749736351099No Hit
CTTAAACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT4490.12141369892647576No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCAAAAATCTCGTATGCCGT3910.10572996944376843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTCGAA150.002222259370.011541
GATCGAA150.002224644969.9925629
GCGATGC150.002224644969.9925629
CGACTAC150.002224644969.9925648
TAACCGA252.8412542E-669.983111
AGGGGGG201850.066.5770270
CCGTCTA700.064.99309547
ACGTATG600.064.1858940
AAGGGGG217900.061.6570669
CGACTTC800.061.24349248
CCGACTT750.060.6602247
TACACGT352.9614966E-760.0260965
TCTCGAA1050.060.01797538
ACTCGTA1050.060.01797538
AAAGGGG229250.058.6197268
AAAAGGG229800.058.50988867
AAACTCG1200.058.3508136
AAAAAGG230800.058.33218866
AACCGAG308.380888E-658.3192512
AAAAAAG231800.058.2617265
Produced by FastQC (version 0.11.5)