FastQCFastQC Report
Thu 26 May 2016
SRR1781471_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781471_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316702
Sequences flagged as poor quality0
Sequence length76
%GC84

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21766568.72864711937406No Hit
CTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGTG50871.6062418298589842No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTCTTGAGTGTAGATCTCGGT3860.12188113747308195No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCAAT150.00222143870.01565612
GCGGCAA150.00222143870.01565611
GGTATAC150.002225621169.9824524
GTACCTA252.84847E-669.9492933
TTGTGCG150.00223120869.938242
AAAAAGG5550.059.97072270
CGTATCA7050.059.1192756
TCGCCGT7200.058.8232952
CGCCGTA7150.058.7451153
GCCGTAT7100.058.6843754
CCGTATC7150.058.28320755
GTGGTCG7450.058.24043348
TGGTCGC7350.058.0898649
GTCGCCG7300.058.00832751
ATCTCGG7250.057.87992542
GTATCAT7250.057.49748657
TATCATT7200.057.4193458
CGGTGGT7500.057.3765546
ATCATTA7250.057.02334259
GATCTCG7300.057.00445641
Produced by FastQC (version 0.11.5)