FastQCFastQC Report
Thu 26 May 2016
SRR1781471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences316702
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT17076353.91914165366811No Hit
TCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG128224.048600892952997No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC67572.1335514142632506No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG64422.0340888279834037No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC25810.8149616990104263No Hit
ATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCTT23540.7432854860405049Illumina PCR Primer Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC18850.5951967464682888No Hit
ATTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT11360.3586968190917645No Hit
CTTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG10860.34290910698385235No Hit
CTTTACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC7800.24628830888342984No Hit
TTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTC7730.24407802918832217No Hit
CTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT7170.22639579162746054No Hit
CTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG5960.18818952832631305No Hit
TATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGTCT5450.17208606197624265No Hit
CCTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCG5070.1600874007742294No Hit
CATATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT4700.1484044938143744No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCC4600.14524695139279195No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCACGTATGCCGT3920.1237756629260314No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCGGTCATCTCGTATGCCGT3900.12314415444171492No Hit
CTTAAACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGCCGT3590.11335577293480938No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGACATCTCGTATGCCGT3520.11114549323970169No Hit
CTTATACACATCTCCGAGCCCACGAGACAAACGGTCATCTCGTATGACGT3400.10735644233380276No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAC252.828494E-670.032231
CGAAAGC207.9141E-570.0101141
ACGTTCA150.00222213970.0101131
GCAACCG150.00222213970.0101139
AATGACG301.0208714E-770.0101143
GAACGGT150.00222213970.0101129
TCGTATT150.00222213970.0101140
CTCGAAA207.9141E-570.0101139
CACGTAA150.00222213970.0101139
TCGAAAG150.00222213970.0101140
ATCGAAT150.00222213970.0101139
ACGTCAT252.8338072E-670.010147
TTCCGAC150.002223531269.99905466
CGTAATA150.002223531269.99905448
ACGAACG150.002223531269.99905427
TACGCAT207.926491E-569.9885
ATATACG252.8391296E-669.98810
CGTACAC150.002224924369.9883
CCGACGA150.002224924369.98819
TATACGA252.8391296E-669.98811
Produced by FastQC (version 0.11.5)