FastQCFastQC Report
Thu 26 May 2016
SRR1781470_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781470_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245184
Sequences flagged as poor quality0
Sequence length76
%GC97

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22458391.59773884103367No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG16530.6741875489428347No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGGAA150.002216357370.051360
CGCTGGG150.002219953370.02267516
CGCTGCG252.8288669E-670.02267516
TGCCGGC150.002219953370.02267519
TCTGGCG207.903487E-570.02267511
GTCTGGC150.002219953370.02267510
GCGGACG207.903487E-570.02267520
CTGCGGA207.903487E-570.02267518
TGGCGGT150.002219953370.02267513
TGGCGGC150.002219953370.02267519
TGCGGAC150.002219953370.02267519
TGACGAC150.002219953370.02267519
GTCGGCG150.002219953370.02267551
GGTCGGC150.002219953370.02267550
CGGACGA150.002221753170.0083821
GACGAGC150.002221753170.0083823
GACGAAG150.002221753170.0083823
CGGCTGG150.002221753170.008389
GTGGTGC150.002225355569.979791
GGAGATC150.002230767669.9369538
Produced by FastQC (version 0.11.5)