FastQCFastQC Report
Thu 26 May 2016
SRR1781470_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781470_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245184
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT17753972.41051618376403No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG124335.070885539023753No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC72422.9537000783085356No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC26971.0999902114330462No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19880.8108196293395981No Hit
ATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCTT18700.7626925084834247TruSeq Adapter, Index 11 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC17530.7149732445836596No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG11560.47148264160793524No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATCTCGTATGCCGTCT9980.407041242495432RNA PCR Primer, Index 38 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCAGT8550.3487176977290525No Hit
CTTTACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC8060.3287327068650483No Hit
TTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTC7360.3001827199164709No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCCGT6350.2589891673192378No Hit
CTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT6240.2545027407987471No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG5630.2296234664578439No Hit
ATTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT5570.22717632471939442No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCG5540.22595275385016966No Hit
TATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGTCT4790.195363482119551RNA PCR Primer, Index 11 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCC4570.18639062907856957No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAATCTCGTATGCCGTC4530.18475920125293657RNA PCR Primer, Index 29 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT3880.15824849908640043No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTAAATCTCGTATGCCGT3800.15498564343513443No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTATGCAGT3510.1431577916992952No Hit
CTTATACACATCTCCGAGCCAACGAGACTCAGCTATATCTCGTATGCCGT3470.14152636387366224No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGACGT3330.13581636648394674No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTAGTAAGCAGT3250.13255351083268077No Hit
CATATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTATGCCGT2960.12072565909684156No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGCTATATCTCGTAAGCCGT2750.11216066301226835No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAA252.8266295E-670.0320439
TCCTTAT150.002218779470.032041
TCTAGTG301.0176882E-770.0320438
CTATAAC850.070.0320433
GAAATAT150.002218779470.0320432
TATAACA150.002218779470.0320434
ATAACTC900.070.0320435
ATAACAC150.002218779470.0320435
CAATAAC150.002218779470.0320433
TAACTCG900.070.0320436
GCTAATC353.6834535E-970.0320432
ATAGTAT252.8266295E-670.0320439
CTTATAT150.002218779470.032041
CTTATAA800.070.032041
CTAGTGA353.6834535E-970.0320439
CTTAAAC1250.070.032041
AATAACT252.8266295E-670.0320434
GTTATAC207.898272E-570.032041
TATAGTA252.8266295E-670.0320438
CTCGTAA1250.070.0320439
Produced by FastQC (version 0.11.5)