FastQCFastQC Report
Thu 26 May 2016
SRR1781469_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781469_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228210
Sequences flagged as poor quality0
Sequence length76
%GC98

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20982391.94294728539502No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG14360.629244993646203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTAAA252.813058E-670.0861161
GTATCAG252.813058E-670.0861157
TCAGTAA252.813058E-670.0861160
AGTAAAA301.0119402E-770.0861162
TATCAGT252.813058E-670.0861158
GGTATCA150.00221179670.0861156
GTAAAAA301.0119402E-770.0861163
GACGCGG150.002221449570.0092114
CTGCGGA150.00222338469.9938518
ACGGGTG150.002225319969.97858
CGACGAA150.002225319969.978522
CGCATCT150.002225319969.97857
TGCGCGG150.00222919569.9478155
TGGAGAT150.00222919569.94781537
ATTAAAG150.002231134869.932483
CGTATCA1800.062.29876756
GTCGCCG1750.061.9809651
GGTCGCC1750.061.9809650
CCGTATC1700.061.8135355
GCCGTAT1700.061.79995754
Produced by FastQC (version 0.11.5)