FastQCFastQC Report
Thu 26 May 2016
SRR1781469_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781469_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228210
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT17142075.11502563428422No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG118215.179878182375882No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC69773.057271811051225No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC23651.0363261907891854No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16830.7374786380964901No Hit
ATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCTT16710.7322203233863547TruSeq Adapter, Index 9 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC14460.6336269225713158No Hit
CTTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG10800.47324832391218613No Hit
CTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT6980.30585863897287585No Hit
CTTTACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC6970.30542044608036456No Hit
TTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTC6590.2887691161649358No Hit
ATTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT5590.24494982691380748No Hit
CCTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG5230.22917488278340123No Hit
CTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCG4970.21778186757810788No Hit
TATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGTCT4220.18491740063976161No Hit
CTTATACACATCTCCGAGCCCACGAGACGTAATCGGATCTCGTATGCCGT4060.17790631435958107No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT4050.1774681214670698No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGATCTCGTATGCCGTC3880.170018842294378RNA PCR Primer, Index 42 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCC3480.15249112659392663No Hit
CTTATACACATCTCCGAGCCAACGAGACGTATTCGGATCTCGTATGCCGT3200.1402217256036107No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTAGGATCTCGTATGCCGT3180.13934533981858815No Hit
CATATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT3100.13583979667849788No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATATCGTATGCCGT2970.13014328907585118No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCAGT2840.12444678147320451No Hit
CTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGACGT2680.11743569519302396No Hit
CTTATACACATCTCAGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT2370.10385171552517419No Hit
GTTATACACATCTCCGAGCCCACGAGACGTATTCGGATCTCGTATGCCGT2310.10122255817010647No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGATC750.070.05131533
ACTCGAA150.002216161570.05131538
ATCTCGA750.070.05131537
ATAAGGA207.8863406E-570.05131531
GATATAG207.8863406E-570.05131536
AGGATCT800.070.05131534
AGGATCA207.8863406E-570.05131534
TCGAATC301.01543264E-770.05131533
TACGGAT600.070.05131532
CTCTCGT252.8213854E-670.05131537
TATCGAA150.002216161570.05131538
TTCGAAT301.01543264E-770.05131532
GGATATA207.8863406E-570.05131535
TCTCGAA750.070.05131538
ATACGGA700.070.05131531
ATCGGAA301.01543264E-770.05131532
GGCTCTC252.8213854E-670.05131535
ATTCGGC301.01543264E-770.05131531
CGAATCT301.01543264E-770.05131534
ATTCGAA301.01543264E-770.05131531
Produced by FastQC (version 0.11.5)