FastQCFastQC Report
Thu 26 May 2016
SRR1781468_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781468_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245143
Sequences flagged as poor quality0
Sequence length76
%GC98

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG22372791.26387455485165No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG15580.635547415182159No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2800.11421904765789763No Hit
GGGGGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2650.10810017010479597No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2500.10198129255169432No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGGCG252.8255254E-670.0365911
GTGTGGC252.8255254E-670.0365910
TCGGCGT150.002218203670.0365852
GGCTGCC150.002218203670.0365816
CGGCGTA150.002218203670.0365853
CGGCGAG150.00222000370.0222822
CGGCCGG252.8289596E-670.0222718
GGCGAGC150.002223604869.9936723
GCATCTG150.002223604869.993678
GTGTCGG207.9438E-569.950842
TAAACAA150.00222901669.95085
TGCGCGT150.00222901669.95085
TCGCGGG150.00222901669.950843
GCGCGTC150.00222901669.95086
GATCGCG150.00222901669.950841
AGGTGTC150.00222901669.950840
CGGGTGT150.00222901669.95087
TACGCAT150.00222901669.95085
ATCGCGG150.00222901669.950842
CGCATCT150.00222901669.95087
Produced by FastQC (version 0.11.5)