FastQCFastQC Report
Thu 26 May 2016
SRR1781468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781468_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245143
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT18125573.93847672582942No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG126425.156990001754078No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC72842.9713269397861657No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC22320.9104889799015269No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18950.7730181975418429No Hit
ATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCTT17610.7183562247341349TruSeq Adapter, Index 2 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC15250.6220858845653353No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG11760.47972000016317007No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGATCTCGTATGCCGTC10240.4177153742917399No Hit
CTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT7560.30839142867632363No Hit
TTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC7260.2961536735701203No Hit
CTTTACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTC6910.2818762926128831No Hit
ATTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT6440.26270380961316453No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG5960.24312340144323924No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCG5110.20844976197566317No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT4560.18601387761429045No Hit
TATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGTCT4440.1811187755718091No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCC3680.15011646263609402No Hit
CATATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT3580.14603721093402627No Hit
CTTATACACATCTCCGAGCCAACGAGACGGGAATGAATCTCGTATGCCGT3350.13665493201927037No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGACGT3290.13420738099802973No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATATCGTATGCCGT3060.12482510208327384No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCAGT2840.11585074833872473No Hit
CTTATACACATCTCAGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT2830.11544282316851796No Hit
CTTATACACATCTCCGAGCCCACGAGACGGGAAAGAATCTCGTATGCCGT2820.11503489799831118No Hit
CTTATAAACATCTCCGAGCCCACGAGACGGGAATGAATCTCGTATGCCGT2580.10524469391334855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTAT150.002219034170.030011
CTTTACA800.070.030011
AAATCGT353.6852725E-970.0300137
GGATCTC150.002219034170.0300135
TATCGAA301.0178883E-770.0300138
CAATCTC207.899403E-570.0300135
ACGTAAG150.002219034170.0300140
ACTCTAG150.002219034170.0300136
CACGAAT353.6852725E-970.0300139
TAATGCC150.002219034170.0300142
CTTAGAC207.899403E-570.030011
TCAATCT252.8271152E-670.0300134
CTGATAC207.899403E-570.030011
AGAATGC252.8271152E-670.0300141
AGTATGA353.6852725E-970.0300141
AACTCGA150.002219034170.0300137
TCACGAA207.899403E-570.0300138
ATAGTAT207.899403E-570.0300139
ATTAATC150.002219034170.0300133
AGAAACT207.899403E-570.0300134
Produced by FastQC (version 0.11.5)