FastQCFastQC Report
Thu 26 May 2016
SRR1781467_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781467_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243431
Sequences flagged as poor quality0
Sequence length76
%GC97

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21971590.25760893230526No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG12790.5254055563999654No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCATGA150.002211720470.0881459
CAGTAAA207.86687E-570.08813561
AGTAAAA207.86687E-570.08813562
GTGTCAT150.002215336370.0592957
CGGTGTC150.002217145970.0448755
CGCCGGA207.8990284E-570.0304553
CGGCCGA150.002220768570.01604518
GGACGCT150.002220768570.01604513
GACGAGC150.002222581370.0016523
CGACGGC150.002222581370.0016522
GTAGATA150.00222984469.9441138
TAGATAT150.00222984469.9441139
TGCCGGC352.961442E-760.01375219
GTGTGGC352.961442E-760.01375210
CATGAAA252.3724744E-456.07050761
GCAGGAA252.3724744E-456.07050760
GAGCAGG252.3724744E-456.07050758
AGCAGGA252.3724744E-456.07050759
TCAGTAA252.3724744E-456.07050760
TCATGAA252.3724744E-456.07050760
Produced by FastQC (version 0.11.5)