FastQCFastQC Report
Thu 26 May 2016
SRR1781467_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781467_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243431
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT17498771.8836138371859Illumina PCR Primer Index 6 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG116694.793555463355119No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC67992.7929885676023187No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC23710.9739926303552136No Hit
ATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCTT22540.9259297295742942Illumina PCR Primer Index 6 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC19340.7944756419683606Illumina PCR Primer Index 6 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16380.6728806109328722No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG12160.4995255329025473No Hit
CTTTACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC8380.3442453919180384Illumina PCR Primer Index 6 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTC8200.3368510994902046Illumina PCR Primer Index 6 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAGGCCAATATCTCGTATGCCGT6940.28509105249536826TruSeq Adapter, Index 6 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATCTCGTATGCCGTCT6810.27975073018637725TruSeq Adapter, Index 4 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT6760.2776967600675345Illumina PCR Primer Index 6 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG5580.22922306526284658No Hit
TATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGTCT5480.22511512502516115Illumina PCR Primer Index 6 (95% over 23bp)
ATTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT5390.22141797881124425Illumina PCR Primer Index 6 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCG5190.2132020983358734No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCC4790.19677033738513175No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCAGT4700.19307319117121485No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAAATCTCGTATGCCGT3950.16226363938857416No Hit
CTTAAACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT3910.16062046329350002Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAAATCTCGTATGCCGTC3460.1421347322239156No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGCAAATATCTCGTATGCCGT3360.1380267919862302No Hit
CTTATACACATCTCCGAGCCAACGAGACCGGCCAATATCTCGTATGCCGT3190.13104329358216496TruSeq Adapter, Index 6 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGACGT3100.1273461473682481No Hit
CATATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT2990.12282741310679411Illumina PCR Primer Index 6 (95% over 21bp)
TCTATACACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT2730.11214676848881203Illumina PCR Primer Index 6 (95% over 21bp)
CTTATAAACATCTCCGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT2610.10721724020358951Illumina PCR Primer Index 6 (95% over 21bp)
CTTATACACATCTCAGAGCCCACGAGACCGGCCAATATCTCGTATGCCGT2510.10310929996590411Illumina PCR Primer Index 6 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGACA252.8289815E-670.021992
AAACTCG150.00222002470.0219936
TCAAGTA207.903777E-570.0219938
ACTCGAA301.01867045E-770.0219938
TATATAC252.8289815E-670.021993
TCCTATC150.00222002470.0219940
ACGCATC150.00222002470.021996
CTTAGAC207.903777E-570.021991
TTATATA252.8289815E-670.021992
CTCTATA207.903777E-570.021991
AGTATGA353.6889105E-970.0219941
AACTCGA301.01867045E-770.0219937
GACACAT252.8289815E-670.021995
TACGCAT150.00222002470.021995
TCACGAA207.903777E-570.0219938
ATAGTAT252.8289815E-670.0219939
ATACGCA207.903777E-570.021994
ATTAAAC150.00222002470.021991
ATAGACA150.00222002470.021994
CTAAAAC500.070.021991
Produced by FastQC (version 0.11.5)