FastQCFastQC Report
Thu 26 May 2016
SRR1781466_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781466_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307064
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19752664.32730635958627No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG12220.39796263971028845No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGATAG150.002223939669.9952731
TGCCGGC252.3852097E-456.01449219
CACTTTA200.006926662352.53930769
CCGTATC1600.052.52215655
CCGGCGG200.00694457252.50501621
GCCGAAG200.00694457252.5050169
GTGGTCG1900.051.5586448
CGTATCA1650.050.93057656
GCCGTAT1650.050.93057654
CGGTGGT1900.049.7172646
CGCCGTA1700.049.42455353
GGTGGTC2000.048.98071347
TCGCCGT1650.048.80050352
GTATCAT1950.048.4819957
GATCTCG1850.047.27096641
GTCGCCG1800.046.67874551
CTGCCGG305.8651326E-446.67874518
GTCTGGC305.869853E-446.67112710
TGGTCGC2050.046.09444849
TCGGTGG2050.046.07189645
Produced by FastQC (version 0.11.5)