FastQCFastQC Report
Thu 26 May 2016
SRR1781466_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781466_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences307064
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT15991252.07774275069692No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG107573.503178490477555No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC69322.25750983508324No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC23480.7646614386577391No Hit
ATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCTT20740.6754292264804731RNA PCR Primer, Index 37 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC15700.5112940624755752No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15450.5031524372769195No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG10340.33673761821639786No Hit
CTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT6640.21624156527629418No Hit
TTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC6320.20582028502201494No Hit
CTTTACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTC6030.19637599979157438No Hit
CTTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG5070.16511215902873666No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCG5010.1631581689810593No Hit
ATTATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT4810.1566448688221348No Hit
TATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGTCT4690.15273688872678007RNA PCR Primer, Index 37 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCC3940.1283120131308131No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT3870.12603235807518953No Hit
CATATACACATCTCCGAGCCCACGAGACATGGGAACATCTCGTATGCCGT3220.10486413255868483No Hit
CTTATACACATCTCCGAGCCCACGAGACATGGGAAATCTCGTATGCCGTC3170.1032358075189537No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGAA207.901686E-570.0316138
AACTCGA207.901686E-570.0316137
TATCACG207.914452E-570.008843
GTCTCCG207.927234E-569.9859910
ACCGAGA150.002225112269.9859913
AAGCGGG207.927234E-569.9859969
CCGAGAA150.002225112269.9859914
CCGACTT455.456968E-1269.9859947
TAGGCGT150.002225112269.9859910
CGACTTC401.3460522E-1069.9859948
AGGGGGG179350.067.27396470
CCGTCTA650.064.60245547
CCGTCAT1500.062.98739647
AAGGGGG192050.062.75234269
CGAGACC950.062.6190515
CGAGCAC1350.062.2097715
CGTCTAC750.060.6545348
GACCACG1100.060.4424518
AAAAGCG352.972847E-759.98867
AAAGCGG352.972847E-759.98868
Produced by FastQC (version 0.11.5)