FastQCFastQC Report
Thu 26 May 2016
SRR1781465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260781
Sequences flagged as poor quality0
Sequence length76
%GC90

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20542778.77376035830831No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG11920.4570885148841365No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCC150.002218471170.03573654
AAGGGCT150.002218471170.03573655
GTCGGGC150.002221854670.00883510
CGCGTCT252.8359318E-669.995397
GACGACC150.002223547869.9953923
CGAGTCG150.002230330169.9416735
CAATCCT150.002230330169.9416734
CGGCCGA308.355724E-658.34069418
CAGCGGA252.3861039E-456.0070659
GCGGCCG509.767973E-1056.00706517
TGCGCGT252.3951771E-455.9640735
GTGCGCG252.3951771E-455.9640734
GTTGTGC252.4020001E-455.9318731
GGCACCC200.00691097452.56719668
GTGGCAC200.00691097452.56719666
CGCCGGA200.006932022552.526853
CGACGAA200.006942564652.50662222
ACTGACG200.006942564652.50662211
TCGGGCG200.006942564652.50662211
TGTGACG200.006942564652.50662211
Produced by FastQC (version 0.11.5)