FastQCFastQC Report
Thu 26 May 2016
SRR1781465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260781
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT16340562.65985635456571No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG107374.1172478056300115No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC68722.6351613039293507No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC23120.8865676563860097No Hit
ATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCTT20130.7719120641457775TruSeq Adapter, Index 7 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC16650.6384667594648383No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15180.5820976221427174No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGCCCATCTCGTATGCCGT12410.4758782273248435No Hit
CTTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG10160.3895989355052707No Hit
CTTATACACATCTCCGAGCCCACGAGACAATCGACCATCTCGTATGCCGT9110.34933526598947007No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACAATCTCGTATGCCGT9020.34588409431668715No Hit
CTTTACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC7340.28146222309140617No Hit
TTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTC6400.2454166522867847No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT5540.21243878963574803No Hit
CTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG5540.21243878963574803No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT4920.18866405144546575No Hit
TATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGTCT4730.18137824458070181No Hit
CCTTATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCG4610.17677668235032462No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCC4340.16642316733197587No Hit
CTTAAACACATCTCCGAGCCCACGAGACCATCGACCATCTCGTATGCCGT3470.13306184116174108No Hit
CTTATACACATCTCCGAGCCCACGAGACCATCGACATCTCGTATGCCGTC3250.12462564373938284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAT207.907906E-570.01649532
CACGAAT150.00222089370.01649539
AACGACC500.070.01649530
ACTCGAA150.00222089370.01649538
CTCGAAA207.907906E-570.01649539
AACTCGA150.00222089370.01649537
ACATCGA700.070.01649528
CAATCGC150.00222089370.01649528
CATCACG500.070.01649536
TCGAAAG207.907906E-570.01649540
TCGAAAA301.0195072E-770.01649531
ATCGAAA353.6925485E-970.01649530
AATCGAA500.070.01649529
ACGAATG150.00222089370.01649540
TTTAATT150.002222583670.00307552
TAAGCTT150.002222583670.00307553
CTGATAC301.0208714E-770.0030751
CGAAAGC207.915424E-570.0030741
GAAAGCC207.915424E-570.0030742
AAAGCCG207.915424E-570.0030743
Produced by FastQC (version 0.11.5)