FastQCFastQC Report
Thu 26 May 2016
SRR1781464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252040
Sequences flagged as poor quality0
Sequence length76
%GC94

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21664685.95699095381686No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG15850.6288684335819712No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCG207.896912E-570.03519416
CGTAGCA150.00221845170.03519456
GCGGATC150.00221845170.03519455
CCGTAGC150.00221845170.03519455
GCCGTAG207.896912E-570.03519454
GGCGGAT150.00221845170.03519454
CGACGAG301.02028935E-770.00735522
TGCGGGC150.00222195370.00735519
CGACGAA252.832774E-670.0073522
GACGAGC150.002223705469.9934523
GACGAAG150.002223705469.9934523
CAGATCT150.002225458969.979547
TGCGGGT252.8495433E-669.937855
ATACACG150.002232483669.9239654
CGTGGGC150.002234242269.910091
GTGGCGC308.3461055E-658.35106312
GTCGGCG308.3461055E-658.35106351
CTGCCGG308.35595E-658.33946218
GTCGCCG2950.058.1532651
GGTCGCC2950.058.141750
Produced by FastQC (version 0.11.5)