FastQCFastQC Report
Thu 26 May 2016
SRR1781464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252040
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT17479069.35010315822885No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG125394.975003967624186No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC80973.2125853039200125No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC22680.899857165529281No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19260.7641644183462942No Hit
ATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCTT17190.6820345976829074RNA PCR Primer, Index 36 (95% over 21bp)
CTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC13370.5304713537533725No Hit
CTTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG11590.45984764323123317No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACAATCTCGTATGCCGT11140.4419933343913665No Hit
CTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT7440.2951912394857959No Hit
CTTTACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC6640.26345024599269956No Hit
CCTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG6170.24480241231550548No Hit
TTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTC6030.2392477384542136No Hit
ATTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT5960.23647040152356769No Hit
CTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCG4930.19560387240120614No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACATCTCGTATGCCGTC4600.18251071258530394No Hit
CTTAAACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT4170.16544992858276464No Hit
TATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGTCT4160.16505316616410093No Hit
CATATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT3500.13886684653229647No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCC3180.12617044913505793No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGACGT2840.11268052690049199No Hit
CTTATACACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCAGT2690.10672909062053643No Hit
CTTATAAACATCTCCGAGCCCACGAGACGGTAAACCATCTCGTATGCCGT2590.10276146643389938No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT150.002217839270.04011
TATATAC207.9097386E-570.012313
ACGCATC207.9097386E-570.012316
CAGCGTC150.002221334570.012312
AGCGTCA150.002221334570.012313
CGACACG207.917519E-569.9984123
CGACAAC207.917519E-569.9984148
AGGGGCG150.002223083769.9984170
ACACGGT207.917519E-569.9984125
AACGTAT207.917519E-569.9984139
ACTCGAA150.002223083769.9984138
TGTCCGA252.8349386E-669.9984111
TAACGTA150.002223083769.9984138
CAACTCG850.069.9984136
GTCCGAG252.8349386E-669.9984112
TGCTGCA150.002223083769.9984134
GAGCCGA150.002223083769.9984116
CAACAGC150.002223083769.9984151
TCAACGA301.02119884E-769.9984111
AAGGGCG207.917519E-569.9984169
Produced by FastQC (version 0.11.5)