FastQCFastQC Report
Thu 26 May 2016
SRR1781463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381702
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23566161.739524550565626No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG16940.4438017091867478No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGCG150.002229982569.950717
ACGACTA150.002231142569.94153628
GACGAAC308.378325E-658.32283823
TATACGC252.4019969E-455.938573
AGATCGA200.00691384152.56639562
CAGATCG200.00691743452.55949461
GCCGTAG200.00692462352.5456954
GTTAGCC200.006935417752.52553
GTGATGC200.00696065452.4767889
TTATACG200.006971490652.456152
CGGGGTA200.006975105552.44927634
TGCCGGC552.2755557E-950.91996819
CGTATCA3150.050.0566656
CGCCGTA3150.050.0238153
CCGTATC3100.049.73371555
TTGTGCG506.227674E-848.9590762
GTATCAT3150.048.9442957
TCGCCGT3250.048.47825652
GTCGCCG3250.048.46553851
TATCATT3200.048.18586358
Produced by FastQC (version 0.11.5)