FastQCFastQC Report
Thu 26 May 2016
SRR1781463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences381702
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT19029049.8530267066979No Hit
TCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG128953.378289870108095No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC83632.190976206569523No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC28800.7545153025134791No Hit
ATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCTT23110.6054461333710591RNA PCR Primer, Index 36 (95% over 23bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20210.5294706341596324No Hit
CTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC19040.49881844999502234No Hit
CTTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG12130.3177871742877952No Hit
CTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT8230.21561322707242822No Hit
CTTTACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC8030.21037353747164017RNA PCR Primer, Index 36 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTC8020.2101115529916008No Hit
CTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG6220.16295434658450833No Hit
CCTTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCG6110.1600725173040749No Hit
TATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGTCT5820.15247496738293223RNA PCR Primer, Index 36 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT5590.14644932434202598No Hit
CTTATACACATCTCCGAGCCCACGAGACTAACAACATCTCGTATGCCGTC5560.14566337090190778No Hit
CTTAAACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT4620.12103682977820393No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCC4340.11370126433710066No Hit
CATATACACATCTCCGAGCCCACGAGACTAACAACAATCTCGTATGCCGT3830.10034005585509115No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGGTA150.002222425570.0106257
GTCCACC150.002222425570.010621
ATCGTAA252.8370105E-670.0014439
AATCCGT150.002224736669.9922744
GACGTAT150.002224736669.9922746
GACAACG207.9261794E-569.9922718
CGTCTAA207.9261794E-569.9922748
CCGTCTA800.069.9922747
AAGCCGA207.9261794E-569.9922744
CGTAATA150.002224736669.9922748
CCGAAAA150.002224736669.9922752
CGATACT150.002224736669.9922723
TCGAATC150.002224736669.9922740
AGGGGGG216500.067.2604770
ACTCGTA1150.063.93948738
TCGAATG1000.062.99304640
ATCTCGA950.062.65749737
CGTCTAC950.062.62466448
ATGACGT1900.062.62466444
CGTATTC453.8562575E-1062.21535548
Produced by FastQC (version 0.11.5)