FastQCFastQC Report
Thu 26 May 2016
SRR1781462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293589
Sequences flagged as poor quality0
Sequence length76
%GC83

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19263165.61247185691562No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG11670.3974944565361781No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGTT150.00222019670.0242456
GGACCCG150.002223203270.0003418
TGCCGGG252.3880319E-456.0002719
AATCCGC200.006923354752.5450859
AGCCTAA200.006923354752.5450865
AAATCCG200.006932710852.52713858
TTGGATC200.00693739252.51817714
TGCCAGA200.00693739252.51817713
CCCGTTT200.00693739252.51817757
GGTTATT200.00693739252.51817712
TATGATC200.00694676252.50025623
AGACGAC200.00694676252.50025622
CGCCCTG200.00694676252.5002569
ACTGAAC200.006951450452.49130225
CAACTTT200.006951450452.49130228
AACTTTT200.006956141452.48235329
CGGGACA200.00696083552.47340434
CTGCCGG552.2773747E-950.9093418
TGCCGGC352.0835752E-550.00024419
GCGCTGC506.163464E-849.01696415
Produced by FastQC (version 0.11.5)