FastQCFastQC Report
Thu 26 May 2016
SRR1781462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293589
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT15331052.21925889593956Illumina PCR Primer Index 1 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG101573.45959828195198No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC63942.177874511647235No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC23450.7987356474527316No Hit
ATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCTT18880.6430758645589583Illumina PCR Primer Index 1 (95% over 24bp)
CTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC15810.538507914124848Illumina PCR Primer Index 1 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG14280.48639424501599177No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACACGATCTCGTATGCCGT9950.33890915531576454TruSeq Adapter, Index 1 (95% over 23bp)
CTTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG9780.3331187476370027No Hit
CTTTACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC6470.2203761040093464Illumina PCR Primer Index 1 (95% over 22bp)
TTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTC5690.19380835113032163Illumina PCR Primer Index 1 (95% over 22bp)
CTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT5470.18631488236957106Illumina PCR Primer Index 1 (95% over 21bp)
CTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG5170.17609651587763847No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT5030.17132794484806993Illumina PCR Primer Index 1 (95% over 21bp)
CCTTATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCG4820.1641750883037171No Hit
CTTATACACATCTCCGAGCCCACGAGACCAAACACGATCTCGTATGCCGT4340.14782570191662495No Hit
TATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGTCT4310.14680386526743167Illumina PCR Primer Index 1 (95% over 23bp)
CTTATACACATCTCCGAGCCCACGAGACCCACCACGATCTCGTATGCCGT4030.13726672320829458Illumina PCR Primer Index 1 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCCGT3620.12330162233598671Illumina PCR Primer Index 1 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACCCAACACGATCTCGTATGCC3330.11342386806045186No Hit
CTTATACACATCTCCGAGCCCACGAGACCCAACACGATATCGTATGCCGT3080.10490856265050802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTAAG550.070.0112540
CGATCTA252.833016E-670.0112535
GCTCTTT150.00222183370.01124639
CGGTGTG150.00222183370.01124636
AACGAAC150.00222183370.01124633
CACGTAT301.0204894E-770.01124639
TTCGCCA150.00222183370.01124630
ATCGAAT150.00222183370.01124639
TCACGTA301.0204894E-770.01124638
ACTCGTA750.070.0112438
CACGTAA207.912501E-570.0112439
TTATGCG150.002223335269.9993225
CCGTCTA455.456968E-1269.9993247
CCGAGAA150.002223335269.9993214
ATACGAG252.8358863E-669.9993212
GACGACT252.8358863E-669.9993246
ACGCATC150.002224838169.98746
ATACGCA150.002224838169.98744
AGGGGGG177300.067.37385670
ACACGAA1250.067.2107932
Produced by FastQC (version 0.11.5)