FastQCFastQC Report
Thu 26 May 2016
SRR1781461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317723
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20154563.43418638247782No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG12830.4038108666983504No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTACGA150.002225600669.9826824
GTACGAA150.002225600669.9826825
GATACCC150.00222838269.9606555
CGCCGTA2100.056.68834753
TTATACG252.3913158E-455.9861452
TATACGC252.3969034E-455.959713
CGTATCA2100.055.02971656
CCGTATC2100.055.0210455
GCCGTAT2100.055.0210454
TCGCCGT2200.054.11160752
CAAGTAT200.00692396152.54492665
TGCCGGC407.463568E-752.50354819
AATTTGG200.00694989952.4952771
GCTTATA200.00694989952.4952771
TCTATAG200.00694989952.4952779
TTGTGCG200.00695422952.487012
ATGTACG200.00695422952.4870123
GTGAGCG200.00695422952.4870127
TACTAGT200.00695422952.487012
CGGGTGT200.00695856152.478757
Produced by FastQC (version 0.11.5)