FastQCFastQC Report
Thu 26 May 2016
SRR1781461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences317723
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT16046050.50311120063703No Hit
TCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG115143.6239113945166075No Hit
CTCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC66972.107810891877516No Hit
TCTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC21400.6735426771118238No Hit
ATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCTT19200.6042999719881784TruSeq Adapter, Index 25 (95% over 22bp)
CTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC16870.530965652470863No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16400.5161728927399024No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATCTCGTATGCCGTCT10270.32323753709992664No Hit
CTTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG9730.30624160038775916No Hit
CTTTACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC7220.22724196863305457No Hit
TTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTC6700.21087551105837474No Hit
CTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT6260.19702697003364567No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCAGT5940.18695530383384268No Hit
ATTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT5180.16303509660931065No Hit
CTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG4960.15611082609694607No Hit
CCTTATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCG4810.15138973256578842No Hit
TATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGTCT4350.13691171240357167RNA PCR Primer, Index 25 (95% over 21bp)
TCTTAATACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCC4280.13470853542236477No Hit
CTTAAACACATCTCCGAGCCCACGAGACATGATGATATCTCGTATGCCGT3870.1218042131038672No Hit
CTTATACACATCTCCGAGCCCACGAGACATGATGAATCTCGTATGCCGTC3590.1129915051790396No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTA650.070.0042538
GCTAGGT150.002222883370.0042544
ACGTAAG150.002222883370.0042540
TAAATCG150.002222883370.0042536
TATACGT150.002222883370.0042554
TAACTCG500.070.0042536
GCAACGG150.002224271769.9932323
ATGTCCG150.002224271769.9932310
TATTGGG150.002224271769.993237
TACGAGA150.002224271769.9932313
GTCCGAG150.002224271769.9932312
ATACGAG252.8378909E-669.9932312
TATACGA252.8378909E-669.9932311
ACGACAC150.002224271769.9932322
TGTCCGA150.002224271769.9932311
AGGGGGG186150.067.04159570
TCACCGA750.065.3270111
AACTCGT700.065.00394437
TATGACG1350.064.8187543
GATAACT600.064.1705634
Produced by FastQC (version 0.11.5)