FastQCFastQC Report
Thu 26 May 2016
SRR1781460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649066
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG20336131.331328401117915No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG13430.20691270225215927No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA2450.042.87444353
TCGCCGT2450.042.8678352
CGTATCA2500.042.03318456
GTCGGAC350.001252398540.01923854
CCGTATC2750.038.20313355
GCCGTAT2800.037.51803654
GTGGTCG3150.036.65882548
GTCGCCG2950.035.60209351
TGGTCGC2950.035.5938549
AGTCCGA400.00241570135.00872410
GATCGCC400.00241844735.0006189
TCGGTGG3650.034.4918745
CGGTGGT3650.033.546746
ATCTCGG3300.032.84641342
CCCGTCA552.9863E-431.8285712
TCTCGGT3450.031.41830843
CGACGGG450.004308343431.10925722
CTCGGTG3700.030.24512544
GGTCGCC3600.030.14407250
CGACGAA703.4899364E-529.9982122
Produced by FastQC (version 0.11.5)