FastQCFastQC Report
Thu 26 May 2016
SRR1781460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences649066
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT16357425.201443304687043No Hit
TCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG110761.7064520403163932No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC68901.0615253302437657No Hit
ATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTCTT21420.33001266435154514Illumina PCR Primer Index 6 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCC20740.3195360718324485No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG16650.2565224491808229No Hit
CTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC15890.2448133163653619No Hit
CTTTATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCG10380.15992210345326976No Hit
CTTATACACATCTCCGAGCCCACGAGACGATCCAAATCTCGTATGCCGTC10030.1545297396566759No Hit
CTTTACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGTC6620.101992709524147No Hit
CTAATACACATCTCCGAGCCCACGAGACGATCCAAAATCTCGTATGCCGT6590.1015305069130104No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAACG150.002225515469.99168410
CCGAATT150.002225515469.99168447
AGGGGGG189800.066.2302870
AAGGGGG204500.061.45235469
TATGACG1150.060.8717143
CGTATGA1400.060.0113641
AAAGGGG214900.058.59238468
AAAAGGG215500.058.55916267
CCGTCTT209450.058.5129847
TGCCGTC210250.058.51122745
TATGCCG210950.058.48748443
ATGCCGT210800.058.4460844
GCCGTCT210200.058.37529446
CGTCTTC210450.058.35134548
CTCGTAT212150.058.29010839
GCTTGAA212500.058.27050456
GTATGCC211750.058.2589642
TCTCGTA212350.058.2561938
TGCTTGA211550.058.22990455
CTTGAAA212800.058.22573557
Produced by FastQC (version 0.11.5)