FastQCFastQC Report
Thu 26 May 2016
SRR1781459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1781459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367954
Sequences flagged as poor quality0
Sequence length76
%GC77

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG21051757.21285812900525No Hit
CTTATACACATCTGACGCTGCCGACGACATTCGATGTGTAGATCTCGGTG14300.3886355359637346No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCG252.8364302E-670.002992
CGGAGTT207.92512E-569.9934748
CGCCGTA2700.059.62407353
CGTATCA2750.058.55591656
GTCGCCG2750.058.53999351
TCGCCGT2800.057.4946452
TGGTCGC2800.057.4946449
CCGTATC2850.056.50132455
GCCGTAT2850.056.4936454
ATCTCGG3300.056.19923842
GTGGTCG3000.055.99477848
TCTCGGG252.3915994E-455.98716743
ATGCACG252.3915994E-455.9871674
GATCTCG3200.055.76846741
CGGTGGT3350.055.36044746
TCTCGGT3100.055.30990243
GGTCGCC2950.054.57118650
TCGGTGG3550.053.22723845
CTCGGTG3300.053.0181544
GTGGCAT200.006947123452.50224354
Produced by FastQC (version 0.11.5)